Fig. 4, Step 3
FTA Test for Antibodies Against Treponema pallidum

Fluorescent anti-human gamma globulin (anti-HGG) is added to the well. (Anti-HGG is an antibody made by another animal against human IgG antibodies. A fluorescent dye is then attached to the antibody.) The anti-HGG will with any human IgG antibodies bound to the Treponema pallidum on the slide. All unbound anti-HGG is then washed from the slide. When viewed with a flourescent microscope, the spirochetes will fluoresce.
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Microbiology Laboratory Manual by Gary E. Kaiser, PhD, Professor of Microbiology
is licensed under a Creative Commons Attribution 4.0 International License.
Last updated: September, 2017